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KMID : 0861020110260040125
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Korea Journal of Herbology
2011 Volume.26 No. 4 p.125 ~ p.132
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Effects of Curculiginis Rhizoma on anti-thrombotic activity
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Roh Seong-Soo
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Abstract
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Objectives: An aim of study is to investigate effects of curculiginis rhizoma in vitro (factor Xa (FXa) inhibitor
assay, prothrombinase assay, prothrombin time (PT) assay, activated partial thromboplastin time (aPTT) assay)
and in vivo experiment (blood clotting time, thromboxane B2 content assay in serum and weight of thrombus
by AV-shunt rat model).
Methods: We gained a human serum and used serum in vitro study such as factor X activity (FXa) inhibition,
prothrombinase inhibition, prothrombin time (PT) and activated partial thromboplastin time. Fifteen SD rats
were divided into three groups (intact control group and two experimental group treated with extract of
Curculiginis Rhizoma(ECR)). Rats were orally administrated DW (intact control group), 600 mg/kg concertration
of ECR and 200 mg/kg concertration of ECR. After one hour, we anesthetized rats and made arteriovenous
(AV) shunt rat models to study weights of thrombus, took a hole blood to study content of thromboxane B2
and blood clotting time.
Results: In vitro, ECR increased a inhibitory activity of FXa, prothrombinase and aPTT compared than intact
control group. Especially ECR made significant increase of FXa and prothrombinase inhibitory activity (p<0.05,
p<0.01). And PT were increased in ECR control group compared with intact control group. In vivo, a blood
clotting time of experiment group treated with ECR 600 mg/kg were significantly increased compared with that
of intact control group (p<0.05) and content of thromboxane B2 was significantly decreased in group treated
with ECR 600 mg/kg in seum. The weight of thrombus were significantly reduced in group treated with ECR
600 mg/kg compared with intact control group (p<0.05). But in vivo experiment study, those of group treated
with ECR 200 mg/kg were reduced compared with those of intact control group without statistical significance.
Conclusions: ECR has a antithromboic activity in internal course with inhibitory activity of FXa and
prothrombinase in vitro, it required to research more study for effective compounds.
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KEYWORD
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Curculiginis Rhizoma, antithromboic activity, factor Xa assay, prothrombinase assay, prothrombin time assay, blood clotting time, thromboxane B2, arteriovenous shunt modle
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